Reliability, Speed And Simplicity Of Nicorandil Determination By High-Performance Liquid Chromatography Reverse-Phase Technique

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Ali Saeed Owayez, Mazin A.A. Najm, Khulood H.Oudah, H. N. K. AL-Salman

Abstract

This study aims to use a high-performance liquid chromatography-ultraviolet technique to assess Nicorandil in drug products using LC100. The experimental estimating procedure also emphasizes the importance of studying the relative stability of Nicorandil in drug products. It conducts a variety of tasks to validate the chromatographic method for estimating Nicorandil in drug products. Analysis of pharmaceuticals obtained from either The purity of the medicine and its composition, if it is concerned with its components, is determined using a synthetic or natural source. The quantitative and qualitative characterization of samples is carried out. In this study, a new method for Nicorandil was developed using RP-HPLC-UV, which is simple, sensitive, accurate, robust, rugged, and quick. The sample was weighed using an electronic balance and a Quantum prominence Isocratic HPLC system along with an L.C. 20 pump, SPD-20A detector, and spinchrom CFR software (column, C18, 250 4.6 mm; 5). A 50:50 mixture of 0.1 percent formic acid and acetonitrile was used to create the versatile stage. A hundred milligrams of the drug were accurately weighed and broken up in methanol to provide one milligram per milliliter. This work aimed to provide a sensitive, exact, and precise RP HPLC technique for analyzing Nicorandil in mass examples or prescription dose configurations. Nicorandil retention was 4.0 minutes over a 10-minute run, with column pressures ranging from 138 to 141 kg/cm2. The lack of extra pinnacles implies that the tablet's active component is not hindered. With a C.V. of less than 1%, the pills were determined to have 99.89 to 100.08 percent of the designated total, indicating the repeatability of the Nicorandil evaluation in the tablet measurement structure. The proposed switching stage HPLC technique was simple, precise, explicit, and less time-consuming.


 

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