Association Of HashimotoʼS Thyroiditis With IRGM Snps (Rs13361189 T/C, Rs4958847 G/A, And Rs10065172 C/T)In Iraqi Arab Patients.

Abstract

Autoimmune thyroiditis including Hashimotoʼs thyroiditis and Graves’ disease has recently been increased globally. The present study aimed to determine correlation between IRGM SNPs (rs13361189 T/C, rs4958847 G/A, and rs10065172 C/T) with the development of Hashimoto's disease in Iraqi Arab patients. For this purpose, 25 HT patients and 25 healthy subjects were enrolled in this investigation. All of them were subjected to the estimation of free thyroid hormones concentrations (ft3, ft4), TSH, thyroid autoantibodies (TPO, TG). In addition to the molecular detection of IRGM genotypes using Real Time PCR and RFLP techniques. The results found that the mutant alleles (CC and AA) were more prevalent in HT patients (20% and 16%, respectively) than in healthy controls (8% and 4%, respectively). The frequency of the heterozygote allele (TC) of the SNP (rs13361189) was higher in HT patients (48%) than in healthy subjects (40%), whereas the frequency of the heterozygote allele (GA) of the SNP (rs4958847) was equal in both groups. On the other hand, it is clear that all of the HT and healthy controls carried the (rs10065172) SNP's wild type (CC) allele. Moreover, a significant negative correlation was discovered between serum concentrations of thyroid antibodies and the IRGM SNPs (rs13361189 and rs4958847). The current study concluded that these IRGM SNPs are not associated with the development of Hashimotoʼs disease in Iraqi Arab patients.