Purification And Characterization Of Levan From Lactobacillus Gasseri And Its Effect Against Pseudomonas Aeruginosa.

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Shaimaa M.S. Zainulabdeen

Abstract

Purification and characterization of levan from Lactobacillus gasseri, as well as detection of its antivirulence factors against haemolysin production, pyocyanin production, biofilm formation, and swarming of clinical isolates of Pseudomonas aeruginosa from wounds and burns, are the goals of this study. Thin layer chromatography (TLC),1H Nuclear Magnetic Resonance (HMNR), Fourier transform infrared spectroscopy (FTIR)andScanning Electron Microscopy (SEM), were used for levan characterization. Characterizationproved that pure levan is a homopolysaccharide containing solely fructose, at bond area in 868 cm-1 which is typical of carbohydrates with nanosphered structure (24-49) nm. The anti-bacterial activity of purified levanhas been evaluated using the Minimum Inhibitory Concentration (MIC) technique against P.aeruginosa isolates, with concentrations that rising from 1.56 to 400 mg/ml; for all isolates, the MIC was 200 mg/ml. Furthermore, the impactof levan on P.aeruginosa virulence factors was investigated. P.aeruginosa hemolysin production has been reduced after treatment with levan, with inhibition 34.39 %. Pyocyanin production was reduced by levan, with concentrations ranging from 2.30-2.68 µg/ml in comparison to3.84-4.56 µg/ml for the control. Biofilm development was prevented by purified levan at various incubation times (24, 48, and 72 hrs).After 72 hours, the highest level of biofilm suppression had been detected. (86.03%). Also, after 24 h, the greatest inhibition of swarming motility was recoded as 77.27%. Inconclusion, levan purified and characterized from local L. gasserisupressed the develpment and virulent determinants of P. aeruginosa isolated from wounds and burns.

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