Genetic Analysis Of Insulin Growth Factor-1 And Growth Hormonegenes Of Labeo Rohita From Ponds Of Gambat, Sindh, Pakistan

Main Article Content

Bakhtawar Pirzada , Javed Ahmed Ujan , Zuneera Akram , Majeeda Ruk , Maria Khushbakht , Zulfiqar Ali Jumani

Abstract

In the anterior pituitary gland, growth hormone (GH) is an ancestral hormone released periodically by somatotroph cells, under the control of the growth hormone-releasing hormone receptor (GHRHR). IGF-1 causes both hypertrophy (increase in cell size) and hyperplasia (increase in the number of cells) in most tissues, including bone, due to growth hormone stimulation. Growing, reproducing, and lactating features in vertebrates and invertebrates are known to be modulated by genes governing growth hormone (GH), the growth hormone-releasing hormone receptor (GHRHR), and the insulin-like growth factor 1 gene (IGF1). Labeo rohita (Fish) from ponds in Gambat Sindh, Pakistan, studied genetic diversity in the GH-1 regulatory gene and IGF1 genes. The Gh-1 and IGF1 genes of Labeo rohita were assessed for genetic polymorphism using blood samples, PCR, and a Nanodrop spectrophotometer. The PCR product was genotyped using Gel electrophoresis and DNA sequencing (Fish). This study found a 01-nucleotide mutation in the IGF-1 gene of Labeo rohita fish. The Gh1 gene in Labeo rohita fish was found to be unaffected by mutations. These changes are classified as a missense point mutation because of how they occurred: a truncated TTA codon was changed to an inert TTC codon, which codes for the essential amino acid leucine, while TTC codon codes for phenylalanine, a vital amino acid that is nonpolar and neutral owing to the inert and hydrophobic nature of the benzyl side chain. It is envisaged that our findings will serve as a guide and reference for selecting high-quality Labeo rohita for industrial usage and genetic development activities in fish based on our findings in this study.

Article Details

Section
Articles