Antimicrobial Activities, Antioxidant Andphytochemical Analysis Of Leaves And Stems Extracts Of Psidium Guajava

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Dipesh kumar Patel , Itisha Joshi , Falaknaaz Shaikh

Abstract

To determine the antioxidant, antimicrobial activity and phytochemical analysis of Psidium guajava leaves and stems extracts against bacteria (Staphylococcus aureus and Pseudomonas aeruginosa) and fungi (Fusarium sp. and Aspergillus niger). The Guava leaves and stems were extracted by aqueous (hot and cold water) as well as solvent (methanol and n-hexane) extraction. The efficacy of these extracts was tested for antimicrobial activity against those bacteria and fungi through a well-diffusion method employing 50 μl leaf and stem extract solution per well. The quantitative analysis of phytochemical was done by using desire solvent; antioxidant activity was determined by 2, 2’-1-picrylhyrazyl (DPPH) radical scavenging method. According to the findings of above assays, all extracts of the guava leaves and stems showed inhibitory activity against such microorganisms. The hot extract of leaves had an antibacterial activity with mean zoon of inhibition of 13 mm. Cold extract of leaves had 4 mm inhibition whereas, absence of inhibition in stems’ cold extraction, against Staphylococcus aureus and Pseudomonas aeruginosa. The phytochemical analysis saw the presence of alkaloids, flavonoids, steroids, terpenoids, tannins, phenolics. This plant contains the high amount of phenolics. This study provides scientific understanding to further determine the phytochemicals, antioxidant and antimicrobial values, investigate other pharmacological properties.

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