Effect Of Ionizing Radiation Cesium Cs-137 On Isolated Lymphocytic DNA From Leukemia And Healthy Individuals Treated With Vitamin D.

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Abathar A. Jabar, Zainab w. Abdul Lateef , Baydaa H. Abdullah

Abstract

Radiation is defined as the physical process where particles or electromagnetic waves pass through a medium or space. Ionizing radiation consists of either photon-radiation (gamma rays and x-rays) or fast moving sub-atomic particles (beta particles, neutrons, etc.). Gamma rays consist of electromagnetic energy in the form of photons emitted by radioactive nuclides such as caesium-137. Deficiency of Vitamin D is most common worldwide and associated with DNA breaking. This study was aimed to Vitamin D shared ionization radiation by attacking the DNA molecule and Vitamin D may potentiate the indirect effects of radiation.The design of the work was studied in vitro study is simply carried in the laboratory. Lymphocytes were obtained from chronic lymphocytic leukemia patients, known concentrations of lymphocyte cells prepared and different concentrations of synthetic vitamin D (1,25(OH)2D3) prepared. Then the prepared solutions exposed to different time of radiation and different distance, monitored spectrophotometrically by 260/280nm ratio.The vitamin D effect hyperchromasia on lymphocyticDNAreferring to increase in the absorbance of DNA solution, indicating separation of DNA strands, while ionization radiation effect hypochromasia on lymphocyteDNA referred to a decrease in the absorbance of DNA solution indicating DNA damage. The longer the exposure to ionizing radiation and the closer the radioactive source is to the sample, the greater the DNA damage.where it was found that vitamin D reduces the effect of radiation.

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