Inhibition Of Candida Albicans And Streptococcus Mutans (Single And Mix Species) Biofilms By Crude Extract Of Rutaangustifolia

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Shafa Noer, Abinawanto, Boy M.Bachtiar, Anom Bowolaksono

Abstract

Plants from the genus Ruta are known to have many phytochemicals that have potential as drugs and have been widely used as traditional medicines. One of the plants that has the potential to be used as traditional medicine is Rutaangustifolia. This plant has been widely used to cure several diseases traditionally, but scientific research on this plant is still rarely done. Several studies have stated that this plant has an antimicrobial effect, but there are no studies that specifically discuss the antibiofilm effect of this plant. This study is the first study to discuss the effect of Rutaangustifolia extract on physiological biofilm formation. The purpose of this study was to see whether the extract of Rutaangustifolia could inhibit the formation of physiological biofilms on the oral microbes that play the most role in the formation of dental caries, namely Candida albicans and Streptococcus mutans, both in single and mixed species. The method used in this research is Cristal Violet (CV) method for biofilm testing and Total Plate Count (TPC) for viability testing as well as morphological visualization using Scanning Electron Microscope (SEM). The results showed that Rutaangustifolia extract in general could inhibit the formation of biofilms produced by C. albicans and S. mutans in both single and mixed species cultures. The viability test showed that the higher the concentration of the extract, the fewer microbes that could survive.

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