Optimization of Cultivation Conditions for Increasing the Production of Exopolysaccharides of The Lactobacillus Plantarum Eb-2 Strain

Abstract

It is shown in the work that the composition of the medium and the cultivation conditions have a great influence on the biosynthesis of EPS of the L. plantarum EB-2 strain. It was found that the cultivation of the culture under the conditions of a shaker-incubator, in a medium based on curd whey with pH 5.5, at a fermentation temperature of 37°C, with a fermentation time of 48 hours, when using peptone and sucrose, the yield of EPS production increases to 910.3 mg/l, which is 3.6 times more than the EPS production output without selection of fermentation parameters (250.0 mg/l). The results show that the selection of the composition of the environment and the growing conditions make it possible to achieve economic efficiency in the production of new biologically active substances for the pharmaceutical and food industries.