Efficacy of fermented and unfermented cow's milk on the immune status and microbial balance of the gut in laboratory rats

Abstract

The study was conducted in the laboratories of the Department of Food Science at the College of Agriculture, as well as in the animal house of the College of Veterinary Medicine at Tikrit University, for the period from the 1^st of October 2019 until the end of February 2020. The aim of the study is determine the effect of some species of lactic acid bacteria in two groups: the first as (B1) Streptococcus thermophillus+ Lactobacillus bulgaricus+ Lb.acidophilus + Bifidobacterium animalis ssp and the second group of (B2) S.thermophillus + Lb.bulgaricus used in the fermentation of cow's milk and estimating the growth rate parameters, hematological and biological parameters, In addition the balance of the normal microbial flora in the intestine after feeding laboratory rats induced with immune reduction with Sandimmune and raising them for 28 days.Laboratory rats were separated into 8 groups, each of which included 4 animals. T1: negative control, T2: positive control: fed on the main ration and 0.3 ml of Sandimmune, T3: fed on the main ration supplemented with unfermented cow's milk. , T4: fed on the main ration supplemented with unfermented cow's milk and 0.3 of Sandimmune, T5: fed on the main ration of fermented cow's milk from starter B1, T6: fed on the main ration of fermented cow's milk from the starter B1 and 0.3 of Sandimmune, T7: fed on the basic diet supplemented with fermented cow milk from starter B2, T8 fed on the basic diet supplemented with fermented cow milk from starter B2 and 0.3 of Sandimmune.

Oral administration of the immunosuppressant caused a significant decrease in the values ​​of IgG and IgA immunoglobulins in laboratory rats, which were at 1002 and 1986 mg/dL, respectively, compared to their values ​​in the control group, which were 1461 and 3101 mg/dL, respectively. Oral fermented and unfermented milk improved their values ​​to be similar with their values ​​in the control group. Also, the microbial balance of the natural flora in the intestines was disturbed in the case of oral administration of the immunosuppressant, and the number of lactic acid bacteria was at 154 mcg/g. And feeding on fermented milk or feeding on unfermented milk contributed to a positive microbial balance to the flora. normal in the intestine.