The Effect Of 2,4-D And Kinetin On Trigonella Foenum- Graecum L. Callus Using Hypocotyl Segments

Abstract

The present study aimed to induce  callus from fenugreek plant  Trigonella foenum-graecum L. in vitro on (Murashige and Skoog) MS medium after supplying with different combinations of auxin (2,4 Dichlorophenoxy acetic acid) at concentrations (1,2,3) mg/L and cytokinin( kinetin) at concentrations  (1,2,3) mg/L. The results showed that kinetin  2mg/L +2,4-D 2mg/L was the best combination which gave the highest fresh weight of callus compared with other treatments.                                                                                                                                  

The results also showed that enzymatic activity of CAT and SOD increased by increasing the concentrations of hormones . The highest catalase  activity  was at kinetin concentrations and the highest value of SOD was at 2,4-D concentrations . It was also found that the proline concentrations in callus increased by increasing the concentrations of  auxin and cytokinin .