In Vitro detection Total Flavonoid, Antioxidant, Hepatoprotective activity and chemical composition of Plantago laceolata aqueous extract

Abstract

Plantago laceolata considered as an ancient herb promising plants. In this work, The target of the study was designed to estimate total flavonoids, anti-oxidant and chemical composition of  Plantago  laceolata aqueous  extract in vitro. Total flavonoids content was   evaluated in the extract, and it was 317.0000 ± 9.64365mg /ml. also, anti-oxidant activity of Plantago laceolata aqueous extract in vitro was assessed in vitro via evaluation of reductive ability. At all concentrations of plant extract tested (0.02, 0.04, 0.08, 0.16, 0.32 and 0.64 mg/ml) in reductive ability; the absorbance was  increase significantly in all dependent concentration  manner. also, the aqueous  plant extract was  increase significantly The results of chemical composition of plant showed contain Tannins, polysaccharide, Alkaloids ,Flavonoids and Polyphenolic Compounds . Also plant extracts counteract the damage caused by methotrexate  drug through the reduction in liver enzymes  GOT, GPT and ALP in all groups of mice the results of interactions indicated the ability of plant to modulating harmful effects  of the drug in liver and its enzymes when administration to albino mice male in 200mg/kg.