Histochemical Use Of Giemsa In Detecting Tumor Associated Tissue Eosinophilia In Histopathological Biopsies Of Squamous Cell Carcinoma.

Abstract

BACKGROUND:
Tumor associated tissue eosinophilia has been the center of attention of histopathologists because this might be a diagnostic and a prognostic indicator in carcinomas. Various researches have been done regarding the relationship of tissue eosinophilia with the tumor using various stains. The aim of this study was to identify the role of a special stain, giemsa, in detecting tumor associated tissue eosinophilia in tumor microenvironment of histopathological samples of squamous cell carcinoma.
MATERIAL AND METHODS:
In this study, we evaluated 61 paraffin embedded blocks of moderately differentiated squamous carcinoma and 17 of poorly differentiated squamous cell carcinoma. Each block was processed and was stained with special stain, giemsa. Each slide was viewed under microscope and tumor associated tissue eosinophils were counted in 10 high power fields by two observers.
RESULTS:
The tumor associated tissue eosinophilia was higher in cases of moderately differentiated squamous cell carcinoma than poorly differentiated squamous cell carcinoma. However, no significant relationship was found between the macroscopic features of the tumor and the number of eos/10HPF.
CONCLUSION:
It is concluded that there is increased number of tumor associated tissue eosinophils in MDSCC than PDSCC.