Variation In Antioxidant Activity And Phyto-Constituents In Different Parts Of Pyracantha Crenulata Collected From Middle Hill Climatic Condition Of Western Himalayas
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Abstract
The variation in antioxidant activity and phyto-constituents of aqueous and alcoholic extracts of different plant parts (i.e. Leaves, roots, stems, and fruits) of Pyracantha crenulata was determined and the correlation between phyto-constituents and antioxidant activities was also explored. The result revealed that the alcoholic extract of leaves exhibited maximum antioxidant activity with the lowest IC50/EC50 value by ABTS (0.029 mg/mL), DPPH (0.047 mg/mL), and PFRAP (0.025 mg/mL) followed by the roots of the plant. A similar pattern was observed in their phyto-constituents, i.e., the leaves exhibited maximum phenolic (127.49 mg/g), flavonoid (23.20 mg/g), and tannin contents (152.32 mg/g) followed by the roots. Similarly, the aqueous extract of P. crenulata leaves showed the highest antioxidant activity and phyto-constituents followed by roots. The correlation analysis revealed that the phyto-constituents exhibited a significant negative correlation with the IC50/EC50 value and the IC50 value of ABTS assay had a significantly positive correlation with the IC50 and EC50 value of DPPH and PFRAP assay, respectively. The study revealed that the alcoholic extract of the plants possesses a significantly higher antioxidant activity and phyto-constituents than the aqueous extract. Further, this difference could attribute to an uneven distribution of phytochemical contents in the different parts of the plant and, therefore varying its antioxidant activity.