Variation In Antioxidant Activity And Phyto-Constituents In Different Parts Of Pyracantha Crenulata Collected From Middle Hill Climatic Condition Of Western Himalayas

Main Article Content

Anchala Guglani, Rajeshwar K. K. Arya , H. K. Pandey , Arun Kumar Singh , Dheeraj Bisht

Abstract

The variation in antioxidant activity and phyto-constituents of aqueous and alcoholic extracts of different plant parts (i.e. Leaves, roots, stems, and fruits) of Pyracantha crenulata was determined and the correlation between phyto-constituents and antioxidant activities was also explored. The result revealed that the alcoholic extract of leaves exhibited maximum antioxidant activity with the lowest IC50/EC50 value by ABTS (0.029 mg/mL), DPPH (0.047 mg/mL), and PFRAP (0.025 mg/mL) followed by the roots of the plant. A similar pattern was observed in their phyto-constituents, i.e., the leaves exhibited maximum phenolic (127.49 mg/g), flavonoid (23.20 mg/g), and tannin contents (152.32 mg/g) followed by the roots. Similarly, the aqueous extract of P. crenulata leaves showed the highest antioxidant activity and phyto-constituents followed by roots. The correlation analysis revealed that the phyto-constituents exhibited a significant negative correlation with the IC50/EC50 value and the IC50 value of ABTS assay had a significantly positive correlation with the IC50 and EC50 value of DPPH and PFRAP assay, respectively. The study revealed that the alcoholic extract of the plants possesses a significantly higher antioxidant activity and phyto-constituents than the aqueous extract. Further, this difference could attribute to an uneven distribution of phytochemical contents in the different parts of the plant and, therefore varying its antioxidant activity.

Article Details

Section
Articles