Studying Activity Of Gene Of Ebv Virus In The Biopsy Of Nasopharyngeal Cancer (Npc)

Abstract

Epstein-Barr virus (EBV) provokes the development of latent infection and is related to a number of malignant neoplasms in humans, which are considered to appear as a result of deregulation of the different stages of the virus’s life cycle. A large quantity of microRNA (miRNA) for EBV has recently been described, and it was suggested that their expression may vary between different latent conditions in normal and malignant tissue. To date, however, no equipment has been used to fully and quantitatively test this idea, by defining the expression profile of miRNA EBV in primary infected tissues. We describe here a multiplex analysis of PCR with reverse transcription, which allows to profile 39 of the 40 known mature miRNA EBV.
With this approach represents an exhaustive miRNA EBV profile in nasopharyngeal primary carcinoma tumors (NPC), including estimates of miRNA copies per tumor cell. This is the first comprehensive miRNA EBV profiling in any tumor associated with EBV. Unlike previous offers, shows that miRNA from BART are present in a wide range of copies from ≤103 per cell, in both primary tumors and in widely used cell lines C666-1, from NPC. Finally, we demonstrate that expression miRNA EBV in a widely used line NPC C666-1, with certain qualifications, broadly representative of primary NPC tumors.