Novel Endolichenic Fungi Isolated From Dirinaria Applanata

Abstract

The conduct of this study generally aimed to isolate and characterized endolichenic fungi derived from host lichen Dirinaria applanata. Specifically, it sought to characterize the endolichenic fungi extracted from host lichen Dirinaria applanata. The host lichen was collected at Luquilu, Cabagan, Isabela. The lichen thalli were collected by detaching it from the substrata, placed inside a separate paper bag, and stored in a cool dry place (Stone et al., 2004). Identification of host lichen was accomplished through morphological characterizations including (1) presence or absence of reproductive structures (apothecia), (2) types of branches, (3) color, (4) presence of soralia, and (5) growth form. Identification through thalline spot test (K, K+C, C test) was done with the use of chemical reagents potassium hydroxide (KOH), sodium hypochlorite (C), and a combination of both (KC). The reagents were dropped directly on the exposed medulla and cortex of the lichen, and an immediate color change indicates a positive result. Endolichenic fungi were then isolated from the identified host lichen through surface sterilization. The isolated endolichenic fungi were morphologically characterized in terms of form, elevation, surface color, and underside color. The collected samples were identified and confirmed as Dirinaria applanata. After careful characterization, seven isolated endolichenic fungi were derived from the host lichen.