Frequency Of Vitamin D3 Receptor Gene Polymorphism Among Iraqi Individuals

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Salih Abdul Mahdi

Abstract

VitaminD(VD) has been various functions in different biological actions like homeostasis of calcium, also its play critical role in cell differentiation additional to proliferation in many target organs. All these functions were culminated through the effective style of VD(1,25(OH)2D3), that attached with specific proteins that found in the cell cytoplasm which ererepresentedthe receptorsof vitamin D.Those receptors termed vitamin D receptor (VDR). Different single nucleotide polymorphisms (SNPs) of VDR have been determined by specific enzymatic restriction positions such as rs1544410 BsmI(B/b).There is a closed correlation between VDR gene polymorphism and several human diseases have been registered. Many projects about VDR genotyping wereperformed by single amplification refectory mutation system-polymerase chain reaction (ARMS-PCR) technique. In the present project for the first time, 100 DNA samples of Iraqi individuals were analyzed for VDR gene polymorphism atposition rs1544410 BsmI(B/b) by using of ARMS-PCR system to identify and thento compare the results of current study with other similar studies. Our findings demonstrated that the genotypic frequencies of BB, Bb, bb were 52, 36, 12 % respectively. On theotherhandweobtainedallelic frequencies of B/b were 70 versus30 % respectively. In comparison with present data as a reference, the genotypic and allelic frequency of VDR gene polymorphism with data gained from previous studies, it was found non-significant difference between BsmI(B/b) gene polymorphismof Iraqi population and other nationalities and races such as Japanese and South Africa. Whereas the Caucasian Polish and Iranian population displayed statistically significant difference in VDR BsmI(B/b) gene allele and genotypes frequencies ( P< 0.0001& 0.0009respectively).

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