Characterization Of Extremely Drug-Resistant Pseudomonas Aeruginosa Isolates From Burn Center In Najaf, Iraq

Abstract

Background:P. aeruginosa is indeed a prominent Gram-negative bacteria and opportunism pathogen
that has been associated in several of the life-threatening diseases in recent years. Carbapenems, in
specific imipenem and meropenem, have a broadest spectrum of activity. Resistance to hydrolyzed by
most β-lactamases provides them significant assets in the combat against severe nosocomial infections

Aim of the study: Examinations the incidence of extensive-drug resistant (XDR) and detecting of
carbapenems antibiotic resistant genes among susceptibility profile in P. aeruginosa which isolated from
Najaf burn Center.
Methods: overall of 73 samples were taken from patients at the Najaf Burn Center from the whole
research period. A Kirby-Bauer disk diffusion technique using 17 antimicrobial agents has been used to
examine P. aeruginosa specimens for bacterial resistance. Carbapenems production was initially
screened by using the disk diffusion method , according to the CLSI (2020) and potential Carbapenems
producing isolates were further determined by using disk diffusion method based on the MIC.

Polymerase chain reactions (PCR) were used to identify Carbapenemase genes with in specimens.

Result:There were 24 (32.8%) P. aeruginosa specimens confirmed.The distribution of antimicrobial
sensitivity revealed extensive drug resistance in 7 of the isolates (29%). Based on the findings of
antibiotic susceptibility, Most of these samples were shown to be resistant to at least one carbapenem,
and 6 (25) % of P. aeruginosa were shown to have considerable resistance to carbapenems, extensive
resistance was found in all of these specimens.3 (50%) types of genes encoding carbapenem-inactivating
enzymes (blaNDM , blaSPM-1 and blaOXA-50) were detected in this study Conclusions:Carbapenem resistance has increased, demonstrating the significance of such a challenge as in management of severe infections with P. aeruginosa which is extensive-resistant.