The Effect Of Celiac Disease On The Numbers And Types Of Bifidobacterium Spp. In The Patients Of AL, Muthanna Province-Iraq.

Abstract

Objective: To identify number and types of Bifidobacterium spp. of the celiac disease patients.
Patients and methods: A prospective case series study was conducted at private clinics in Al-Muthanna city during the period from July 2020 to March 2021. A total of 75 patients (35 males, 40 females) and total of 25 healthy individuals ( 13 males, 12 females). The identification of Bifidobacterium sp. was carried out by morphological standard of colonies, microscopic test, biochemical examinationand PCR technique using genus specific primer.One hundred samples (children , adolescents, adults and elderly) from three to fifty five years were enrolled in this study, including seventy five celiac patients and twenty five healthy individuals as control group, during the period from July 2020 to March 2021.
Results:The isolates showed gram positive reaction, they appear under the microscope as short rods, some curved, others Y-shaped, it may be single or dual. Some biochemical tests was done for diagnosisBifidobacterium in pure isolates, all of them were negative for (Catalase, Oxodiase, Gelatinase, Indole production, Voges-Proskauer, and Production of ammonia from arginine). It is found that there is a significant decrease (P < 0.05) in Bifidobacterium counts in the celiac patients gut ( 1.1×105 cfu/gm ) comparing with the Bifidobacterium counts in the control group ( 2.1×109 cfu/gm ).Uniplex-PCR products ofBifidobacterium species using 16SRNA-specific primer. Showed the following species:Bifidobacteriumadolescentis (4), B. angulatum (7) The distribution of bifidobacterial species showed that theB. angulatum(22.7%), and B. adolesceatis(5.3%) in patients group.