Molecular Investigation Of Some Erythromycin Resistance Genes In Staph Aureus Isolated From Different Clinical Infections In Diyala, Iraq

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Zainab Amer Hatem , Hadi R. Rasheed Al-Taai and Abbas Abood F. Al-Dulaimi

Abstract

Staphylococcus aureus is an important infectious pathogen in health sector and communities. It causes various infections ranged between simple to life threating infections. This study was carried out during the period from the beginning of December 2019 to the end of August 2020,. Out of 300 specimens, 75isolates of S.aureuswere recovered..All isolates were tested toward the different class of clinically important antibiotics (15) by using agar diffusion method. The results of resistance were as following: Cefotaxime (100%) ceftriaxone (92%), Imipenem (12%). while the resistance to Fluoroquinolones include Norfloxacin (64%), Ciprofloxacin (56%), Amikacin was (70.6%), Gentamycin (74.6%) ,vancomycin is (48%) ,azithromycin (84%),erythromycin (80%) . tetracycline (88%) but resistance to doxycycline is (60%) ,colistin (100%), Trimethoprim (68%),Chloramphenicol (36%) . In this investigation, antibiotic susceptibility testing of the Saureusisolates showed that 9(12%), 24(32%), 42(56%)of the isolates were MDS, MDR,XDR .The polymerase chain reaction (PCR) assay wasapplied to determine the major erythromycin-resistant genes (ermA, ermB, ermC) . this genes detected in 15 isolates and results show that the ermA, ermB,ermCgene found in 5(33.3%) ,5(33.3%),15(100%) .It is well known that many bacterial species exhibit variable genetic variations as a result of the difference in the clinical source from which they were isolated. Within these bacterial species, Staphylococcus aureus represents a crucial bacterial organism that can be used to connect its genetic variation to the clinical source .This study was conducted to identify the genetic polymorphism of two bacterial samples of S.aureus that were isolated from two clinical sources (wounds and burns) in Diyala province.TheermC gene-based genetic investigation revealed a remarkable deletion in both investigated samples since S12 and S14 showed one deletion mutation of A54del. This deletion manifested in clear phylogenetic positioning for both samples in the ermC gene-based tree

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