Genetic Polymorphism Of Some Cytokines In Newborn Infants With Hypoxic-Ischemic Encephalopathy

Abstract

Background. It is now known that cytokine production directly depends on the genes encoding their activity. The study of the relationship between the polymorphism of these genes and cytokine production will make it possible to predict the risk of pathology development, the nature and severity of its course. The aim of the study was to investigate the polymorphism of cytokine genes IL-1β; IL-10, TNFα in preterm infants with hypoxic-ischemic encephalopathy. Materials and Methods. During the research a total of 112 premature infants with hypoxic-ischemic encephalopathy and 95 healthy preterm infants were examined. Genotyping of polymorphic regions of immune response genes was carried out by polymerase chain reaction (PCR) with allele-specific primers (NPF Litech, Moscow) and electrophoretic detection of reaction products in agarose gel. Clinical, neurosonographic, and statistical studies were performed. Results. The distribution of allele and genotype frequencies of cytokine polymorphisms has been established, indicating that the -308(G/A) TNF polymorphism as well as the IL-1-511T/C TT polymorphism contribute to the predisposition to hypoxic-ischemic encephalopathy (HIE) development in preterm infants. Conclusions: The findings may be one of the prognostic factors for the development of hypoxic-ischemic encephalopathy in premature infants.