Extraction, Purification And Antifungal Activity Of Lectin From Rice (Oryza Satival.)

Abstract

The lectins was extracted from the rice gain by hexane and possessed the ability to agglutinate rabbit erythrocytes indifferent levels with higher
titer 128U/ml of hemagglutination activity. The lectin was extracted by hexane and purified using the fractionation method withammonium salts
followed by loading on DEAE -celluloseion exchangerafter that applied to sephadex G-50 in size exclusivetechnology with 19.33fold of purification
and a yield of 46%. Cladosporium sp., Fusarium sp., Aspergillus sp.and Trichoderma spwere the most fungal isolates that isolated from pepper
plants. The purified lectin showed inhibitory activity against fungal isolates Aspergillus sp.with higher inhibitory activity toward followed by
Fusarium sp.The percent of inhibition for Aspergillus sp. was 40% while 32% for Fusarium sp.In contrast, lower inhibition for Cladosporium sp. and
this lead to increase the benefic with using the lectin as biological control agent for phytopathogenic fungi.