Immune Diagnosis Of Potyvirus Bea Common Mosaic Virus (Bcmv) And Its Biological Resistance With Spirolina Platensis And Some Medicinal Plants On Two Bean Cultivars Under Open Cultivation Conditions

Abstract

This study was carried out in the laboratories of the College of Agriculture, Tikrit University and in the Al-Aswad area of Al-Khalis district / Diyala governorate, the spring loop of the bean plant for the season 2021, where it aimed to diagnose the local isolate of Potyvirus Bea common mosaic virus (BCMV) based on the symptoms it causes on bean plants in the field and those caused on indicator plants (Phaseolus vulgaris, Vigna unguiculata, Vicia faba, Pea (Pisum sativum), and groundnut (Arachis hypogaea) and Medicago sativa all responded to artificial inoculation with virus-infected bean leaf extract. Where the plants infected with BCMV virus showed mosaic symptoms between the veins of the leafs, leaf curling down, deformation and plant stunting. It also aimed to biologically control the virus with some organic food supplements and medicinal plants. The results of the examination with the BCMV Immuno strip showed a positive interaction with Flash Kits containing the anti-virus detection serum, the sedimentation line appeared clearly on the test strip, which indicates the presence of BCMV virus in the bean plants that were grown for the purpose of maintaining the isolation of the virus, and in the plants that detect it as well. It was noted that the treatment of Spirolina alga (S. platensis) was superior in reducing infection with the virus and for all traits compared to the treatment of turmeric, black seed, as it stimulated the self-resistance of plants against the virus through decreasing indicators of infection and severity and increasing growth and yield, especially in the local variety infected with BCMV virus, as it gave the lowest percentage and severity of the infection was 50.50 and 17.43%, respectively, in the field experiment.