EVALUATION OF IN-VITRO AND IN-VIVO IMMUNOMODULATORY ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF PROSOPIS CINERARIA (L.)

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*Ms. Pallavi M. Patil, Dr.Vanita G. Kanase, Dr. Jignyasha A. Rawal, Dr. Dheeraj S. Randive

Abstract

Prosopis cinerariaLinn. is used for rheumatism, cough, common cold, bronchitis, dysentery, leucoderma, leprosy, asthma and scorpion stings. Present work was aimed to study immunomodulatory activity of Prosopis cinerariaL. leaves extract. Both ethanol (PEE) and aqueous extract (PAE) was used for the in-vitro and in-vivo immunomodulatory activity of Prosopis cineraria L. After phytochemical evaluation and acute oral toxicity studies in-vitro immunomodulatory activity was studied by evaluating release of following immune mediators from murine peritoneal macrophages such as superoxide (NBT reduction), nitric oxide (NO), lysosomal and myeloperoxidase enzymes. In-vivo immunomodulatory effect was studied in test systems such as Carbon Clearance Assay, Delayed Type Hypersensitivity reaction, Antibody Titer Assay and cyclophosphamide induced myelosuppression in mice. Results showed that, the phagocytic index of PEE and PAE treated mice was found to be significantly higher than the vehicle control group in dose dependant manner. This in vivophagocytic response would be due to enhanced lysosomal enzyme activity of macrophages as observed during in-vitroassays. Study concluded that, both specific and non-specific immunostimulating properties of the PEE and PAE in in-vivo experimental methods suggest itstherapeutic effectiveness in immunocompromised conditions.

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