Method Development And Validation For Simultaneous Estimation Of Amlodipine Besylate And Olmesartan In Bulk And Pharmaceutical Formulation By Rp-Hplc

Abstract

Objective: To develop and validate the RP-HPLC method for simultaneous estimation of amlodipine besylate and
Olmesartan in bulk and pharmaceutical formulation. Materials and methods: Simultaneous estimation of amlodipine
and Olmesartan were carried out by RP- HPLC using Acetonitrile: Phosphate buffer (pH 4.0) (58:42) and column
Phenomenex Luna C-18 (150×4.6mm, 5μ) as a stationary phase and peak was observed at 235nm which was
selected as a wavelength for quantitative estimation. Results: Method was developed and this method was
validated as per ICH guidelines for specificity, linearity, precision, accuracy, robustness and ruggedness studies.
Conclusion: All the validated parameters were within the limits. The method was found to be suitable for the
estimation of amlodipine besylate and Olmesartan in bulk and pharmaceutical formulation.