IMMERSION EFFECT OF AUTOPOLYMERIZED SOFT DENTURE LINER IN SODIUM HYPOCHLORITE AND Ricinus communis ONCandida albicans COUNTS AND SURFACE ROUGHNESS

Main Article Content

Wennie Fransisca, Ismet Danial Nasution, Urip Harahap, Putri WeldaUtami Ritonga

Abstract

Soft denture liner (SDL) is a soft material based on acrylic or silicon which is used in prosthodontic field as a soft
coating material. SDL has almost the same resilience as the oral mucosa which provide a cushion effect so that it can prevent
the concentration of local pressure and distribute the functional loading evenly on the mucosa and minimizing trauma on the
supporting tissue under the denture area. The SDL that used in the oral cavity can cause an accumulation of plaque which is a
colonization of microorganisms such as bacteria and fungi which create an anaerobic environment. This enviroment can trigger
excessive growth of microorganisms, especially Candida albicans, therefore, SDL must be cleaned periodically with cleaning
agents that have an antimicrobial effect that can eliminate the excessive Candida albicans. The recommended cleaning method
is chemically method by soaking into the cleaning material, but soaking in the cleaning material can cause changes in the
physical properties of the material such as the release of soluble components namely plasticizer from acrylic SDL or the
occurrence of water absorption in the filler of silicone SDL material, this can cause the roughness changes of the surface
material, therefore, the ideal cleansing agent must have a good antimicrobial effectiveness without causing significant surface
roughness changes. The aims of this study to examine the effect of Ricinus communis and sodium hypochlorite cleaning agents
on the amount of Candida albicans and the surface roughness of autopolymerized SDL acrylic and silicone. The research
material used in this study are autopolymerized SDL acrylic and silicone. Types of cleaning agents used in this study are 0.5%
sodium hypochlorite and 10% Ricinus communis. The sample were divided into 4 groups (n = 8), which were autopolymerized
SDL acrylic and silicone groups in 0.5% sodium hypochlorite immersion, autopolymerized SDL acrylic and silicone in 10% Ricinus
communis with tween 80 as an emulsifier immersion. The total amount of Candida albicans colony is count with a colony
counter in CFU / mL, meanwhile for the measurement of surface roughness the Atomic Force Microscope (AFM) was used on
the same surface plane ath the baseline and after immersion in µm units. Based on statistical test results, there was no
statistical significant results between autopolymerization SDL acrylic group in 0.5% sodium hypochlorite immersion and in
Ricinus communis 10% immersion on the amount of Candida albicans colony (p = 0.666) and surface roughness (p = 0.341 ) and
there was also no statistical significant results between the autopolymerized SDL silicone group in 0.5% sodium hypochlorite
immersion and in the immersion of Ricinus communis 10% on the number of Candida albicans colony (p = 0.478) and surface
roughness (p = 0.764). For the surface roughness group, the results of the statistical paired T-tests showed that there was a
statistical significant differences of changes of 3 groups of immersion which before and after immersion of acrylic SDL in 0.5%
sodium hypochlorite, (p = 0.001) before and after immersion of acrylic SDL in Ricinus communis 10% , (p = 0.001) before and
after immersion of silicone SDL in sodium hypochlorite 0.5% (p = 0.02) and there was no difference in the groups before and after immersion of silicon SDL in Ricinus communis 10% (p = 0.095). Based on the study results, it can be concluded that Ricinus
communis 10%was effective as cleansing agent for soft denture liner because of the same antimicrobial effect with 0.5%
sodium hypochlorite which was the gold standard for disinfectant agent and the surface roughness does not exceed 0,2 µm on
both autopolymerized acrylic and silicon soft denture liner.

Article Details

Section
Articles