Comparison Of Enzymatic Activity Of Catechol 1,2-Dioxygenase And Catechol 2,3-Dioxygenase In Pseudomonas Spp. Towards Polycyclic Aromatic Hydrocarbons

Abstract

This study aimed to measure the enzymatic activity of catechol 1,2-dioxygenase (C12O) and catechol 2,3-dioxygenase (C23O) produced by four strains of bacteria include Pseudomonas putida (worod-1and worod-2) Pseudomonas aeruginosa (worod-3 and worod-4) they were submitted to GenBank under the Accession number of MW551536, MW551537, MW551538, MW551539,respectively which isolated from soil contaminated with crude oil and it's derivatives and used for remediation of polycyclic aromatic hydrocarbons.the cleavage of the aromatic ring is the first step in the biodegradation of aromatic hydrocarbons by bacteria. It is catalyzed by C120 and C230 enzymes, the isolates showed the highest activity of C23O than C120 in different concentration of PAHs during one and two weeks of remediation.