Investigation Of Antitubercular Activity Using Alamar Blue Dye, In Vitro Antioxidant Activity Of Different Extracts Of Grewia Tiliaefolia Vahl Leaf

Abstract

In the current study, the antioxidant activity and Anti-TB activity of medically important plant Grewia tiliaefolia
Vahl leaf different extracts were evaluated. Antioxidants present in the plant was a determination by 2, 2
diphenyl- 1-picrylhydrazyl (DPPH), Hydrogen peroxide assay, Reducing power assays, Anti-TB activity using
Alamar Blue Dye. The selected plant extracts showed the concentration-dependent percentage inhibition on the
free radicals. The extracts hexane, ethyl acetate, and methanol showed the concentration-dependent
percentage of inhibition compared with ascorbic, and the 50% Inhibition Conc. (IC50) for DPPH radical 343,
260.79,204.8, 41.89 µg/ml hydroxyl radical 367.8, 324.95, 292.81, 78.47 µg/ml and reducing power380.39,
282.01, 233.91, 44.79 µg/ml respectively. In the Alamar Blue Dye for anti-TB activity, the MIC for standard drugs
was found as Pyrazinamide-3.125µg/ml, Ciprofloxacin3.125g/ml, Streptomycin 6.25µg/ml and 6.25 μg/ml for
both methanol and ethyl acetate extract activity similar to that of standard Streptomycin, 12.5 for hexane
extract.