Cytotoxic Activities Of Ferulago Abbreviata Total Flavonoid Extract On Three Different Cancer Cell Lines

Abstract

The ability of various medicinal plants to generate natural chemicals varies. The total flavonoid compounds and cytotoxic activity of aerial parts of Ferulago abbreviata harvested from Piramagrun Mountain in Iraq's northeast Kurdistan region were investigated for the first time. Total flavonoid compounds (TFCs) were extracted using 10 percent hydrochloric acid. The aluminum chloride technique and high-performance liquid chromatography were used to analyze the plant's total flavonoids extract. This research compared the cytotoxic effects of total flavonoids from F. abbreviata aerial parts on normal epithelial cells (HBL100) against three lines of cancerous human cells: colorectal carcinoma (HCT 116), lung adenocarcinoma (A549), and cervical carcinoma (HeLa). MTT assays were performed to evaluate the cytotoxicity and cell viability of the TFC. HPLC revealed several peaks with varying retention periods; isorhamnetin had the greatest concentration (26.22 μg/mg of extract), while chlorogenic acid had the lowest (0.08 μg/mg of extract). The findings of this research suggest that the cytotoxic effects of F. abbreviata TFC products against A549, HCT116, and HeLa cell lines are linked to apoptosis induction. TFCs, on the other hand, had no discernible impact on the normal epithelial cell line (HBL100). Because of its cytotoxicity, it may be a promising candidate for the creation of chemopreventive drugs that induce death in cancer cells. The current research is the first to look at the cytotoxicity of F. abbreviate aerial parts TFC extracts against the A549, HCT116, and HeLa cell lines.