Antibacterial Activity Of Cloves Seeds (Syzygium Aromaticum) And Cinnamon Bark Essential Oils

Abstract

The antimicrobial activity of the Clove and cinnamon plant essential oils was evaluated by the disc
diffusion method against five pathogens including Staphylococcus aureus, Escherichia, Escherichia coli,
Pseudomonas aeroginosa, Proteus spp., and Acinetobacter spp.
5% solution of DMSO was used to make dilutions for essential oils from 1:2 to 1:8.
Sterile filter paper discs of 5 mm diameter were dipped aseptically in appropriate concentration of
essential oils
and placed over Mueller–Hinton plates seeded with respective pathogens. The plates were incubated
at 37°C for 24 h. After overnight incubation, the diameter of each zone was measured in mm, a n d the
results were recorded according to the size of inhibition zone. The best effect of antibacterial activity
of clove oil was on Acinetobacter spp, the maximum zone of inhibition was 30mm at 1:2 dilution and
minimum zone of inhibition was14mm at 1:8 dilution , and the least on Escherichia coli.
Cinnamon essential oil show strongest antibacterial effect than clove on all the five pathogens. On
Acinetobacter spp the maximum zone of inhibition was 44mm at 1:2 dilution and minimum zone of
inhibition was 30 mm at 1:8 dilution.
On Escherichia coli the zone of inhibition was 32mm at 1:2 dilution and minimum zone of inhibition
was 28 mm at 1:8 dilution.
On staphylococcus aureus as a representative of Gram positive bacteria the zone of inhibition was30
mm at 1:2 dilution and minimum zone of inhibition was 10 mm at 1:4 dilution.
The antibiotics (TS (TRIMETHOPRIM),T(TETRACYCLINE), E (ERYTHROMYCIN) ,CIF (CIPROFLOXACIN), FOX
(CEFOXITIN), SAM (AMPICILLIN), and NA (NALIDIXIc ACID), were used in this study to get a results for
comparing their effect with the clove and cinnamon oils.
The effect of the clove essential oil higher than the effect of antibiotics used against the Acinetobacter
spp. But it is weaker than its effect on the rest of the path genes .
The results of this study confirm that Cinnamon is more active than clove and more potent than all
antibiotics used against all tested bacteria