Production Of Pectinase By Saccharomyces Cerevisiae Using Sugar Beet Pulp

Abstract

Industrial waste management is a great matter of concern nowadays. Industrial waste is generated in the solid, liquid and in gaseous forms and discharged in the landfills. Residual organic compounds generated from raw material of many food industries are having adverse environmental impact. Waste material in the form of peels of fruits and vegetables, seeds of some fruits and some pulp are produce by the food processing industries in large amount. These waste material like sugar beet pulp and soybean powder contain Pectins, which can be used as the substrate for production of pectinolytic enzymes by microorganisms using solid state fermentation technique . In the Present study the yeast strains were isolated from active dry yeast powder and these potential yeast strains were screened for pectinolytic activity on selective media. The yeast strain S. cerevisiae was shown to possess polygalacturonase and pectin lyase activity in solid state fermentation.
This yeast strain was used for their ability to produce pectin’s on sugar beet pulp and soybean powder medium in the ratio 1:9 and 9:1. The highest producer medium was identified as the medium medium containing soybean powder and sugar beet pulp in the ratio of 9:1.
The highest polygalacturonase and pectin lyase activity was observed in media containing ratio of 9:1 by growing the yeast on Soybean powder and sugar beet pulp and incubation for 10 days at 300C. Thus integrated approach towards utilization of waste from fruits and vegetable for pectin’s production by SSF. This is step toward reducing waste and pollution.