Nephroprotective Effect Of Ethanolic Extract Of Cissus Quadrangularis Linn Fruits In Gentamicin Induced Nephrotoxicity: In Vitro Cell Viability And In Vivo Models

Abstract

Nephrotoxicity is known to be a major complication during cancer patients. The present study was undertaken to evaluate nephroprotective activity of ethanolic extract of Cissus quadrangularis linn (EECQ) fruits in Wistar albino rats and in human embryonic kidney (HEK)-293 cells. Ethanolic extract of Cissus quadrangularis linn against Gentamicin induced renal damage was evaluated using in-vitro human embryonic kidney (HEK)-293 cells. Randomly selected animals were divided into five groups of six animals each. The test extracts were administered orally at a dose of 100mg/kg & 200 mg/kg. Gentamicin was administered at a dose of 80 mg/kg i.p. to the rats for 8 days. On eighth day all the animals were sacrificed and blood was collected. Elevation of urea and creatinine level in the serum was taken as the index of nephrotoxicity. Histopathological examinations of kidneys of all the groups were carried out. Co-treatment of HEK-293 cells with Gentamicin and EECQ extract at varying concentrations resulted in significant enhancement of cell growth compared to EECQ treatment indicating the cytoprotective activity of EECQ. The findings also revealed that EECQ possesses nephroprotective activity. The elevations of serum urea and creatinine produced by Gentamicin were considerably reduced and showed histopathological changes in the kidneys to normal. The study concludes that Cissus quadrangularis Linn possess promising nephroprotective activity due to its potent chemical constituents.