Geometrical Correction Method For Simultaneous Estimation Of Azilsartan Medoxomil And Amlodipine Besylate From Biological Sample

Abstract

The geometrical correction method eliminates background irrelevant absorption that may be present in the sample from biological origin. Three-point geometrical correction method was used for quantification of Azilsartan medoxomil and Amlodipine besylate from biological sample. The method was developed at three wavelengths 240nm ,251nm ,262nm for Azilsartan Medoxomil and 226nm 239nm,245nm for Amlodipine besylate. The proposed method shows linear relationship at three wavelengths in the range of 4.4-44μg/ mL for Azilsartan Medoxomil and The concentration rang of 3.4-34 μg/mL for Amlodipine besylate with the regression coefficient of 0.9976 ,0.9961 ,0.9986 for Azilsartan Medoxomil at 251nm,262nm,240nm. and 0.9978 ,0.999, 0.9991 for Amlodipine besylate at 226nm 239nm,245nm. The accuracy at three wavelengths was found to be 98.53 ,98.01 ,99.02 for Azilsartan Medoxomil at 251nm,262nm,240nm. 97.33,98.51,97.5 for Amlodipine besylate at 226nm 239nm, 245nm.The % RSD at three wavelengths was found to be 0.23 ,0.6978 ,0.2374 for ruggedness and 0.5897 ,0.4503 ,0.7745 for robustness of Azilsartan Medoxomil at 251nm,262nm,240nm. and 0.5407 ,0.4923 ,0.2144 for ruggedness and 0.7530 ,0.2459,0.3482 for robustness of Amlodipine besylate at 226nm 239nm,245nm. The proposed method was found to be accurate, precise, rugged and robust. The proposed method was validated as per ICH guidelines. corrected absorbance D value was

calculated. D value showed linearity. Simultaneous estimation of Azilsartan Medoxomil and Amlodipine Besylate with egg albumin was 99.93% and 96%. also Absorbance ratio of Azilsartan Medoxomil and Amlodipine besylate by using egg albumin was found to be 99.93% and 96%