Fatih Demirci1*, Pınar Bayramiç2, Gamze Göger3, Betül Demirci1,3 and Kemal Hüsnü Can Başer 1,4,5
1Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, Eskişehir, Turkey
2Ministry of Health, Directorate General of Health Investments, Ankara
3Graduate School of Health Sciences, Anadolu University, 26470-Eskişehir, Turkey
4Badebio Biotechnology Ltd., Eskişehir, Turkey
5King Saud University, College of Pharmacy, Department of Botany and Microbiology, Riyadh, Saudi Arabia
*Corresponding author. Email: fdemirci@anadolu.edu.tr
FULL TEXT: NVEO 2015, Volume 2, Issue 2, Pages 39-44
Abstract
Pinus pinea L. is commonly known as Stone or Umbrella Pine, which is a member of the family Pinaceae and grows natively in the northern Mediterranean and Aegean coastal regions; southern Europe, north Africa, Spain to Turkey. P. pinea is also cultivated for its edible pine nuts, as ornamental trees and commonly planted in gardens and parks. Its essential oil is used for a variety of skin complaints, wounds, sores, burns, in herbal steam baths and various inhalers. Air dried needles collected from Ortanca-Muğla were subjected to water-distillation using a Clevenger-type system. The resulting essential oil was analysed by GC-FID and GC-MS, simultaneously. Overall, thirty components were characterized. Limonene (54.6 %), α-pinene (4.0 %), myrcene (2.4 %) and α-phellandrene (2.4 %) were characterized as major constituents. The essential oil was also screened against 8 different human pathogenic microorganisms, where the minimal inhibitory concentrations (MIC) were determined using a microdilution method. The oil showed the same inhibitory activity against Escherichia coli, Staphyloccocus aureus, Pseudomonas aeruginosa, Enterobacter aerogenes, Proteus vulgaris and Salmonella thyphimurium (MIC>0.75 mg/ml). Its antifungal susceptibility against Candida parapsilosis was relatively more than that of the pathogen Candida albicans with a MIC value of 0.375 mg/ml, when compared with the antifungal standards.
Keywords
Pinus pinea, GC-FID/GC-MS, limonene, antimicrobial
References
Amri, I., Gargouri, S., Hamrouni, L., Hanana, M., Fezzani, T., Jamoussi, B. (2012). Chemical composition, phytotoxic and antifungal activities of Pinus pinea essential oil. Journal of Pest Science, 85(2), 199-207.
Arshad, M.A., Khan, M.A., Muhammad, Z., Sarwat, J., Shahzia, S. (2010). Ethnopharmacological application of medicinal plants to cure skin diseases and in folk cosmetics among the tribal communities of North-West Frontier Province Pakistan. J Ethnopharmacol 128,322–335.
Bouchra, C., Mohamed, A., Mina, I.H, Hmamouchi, M. (2003). Antifungal activity of essential oils from several medicinal plants against four postharvest citrus pathogens. Phytopathologia Mediterranea, 42(3), 251-256.
ESO. (1999). The Complete Database of Essential Oils, Boelens Aroma Chemical Information Service, The Netherlands.
Hmamouchi, M., Hamamouchi, J., Zouhdi, M., Bessiere, J. (2001). Chemical and antimicrobial properties of essential oils of five Moroccan Pinaceae. Journal of Essential Oil Research, 13(4), 298-302.
Joulain, D., W.A., Koening. (1998). The Atlas of Spectra Data of Sesquiterpene Hydrocarbons, EB-Verlag,Hamburg.
Koenig, W.A, Joulain, D., Hochmuth, D.H. (2004). Terpenoids and Related Constituents of Essential Oils. MassFinder 3. Hochmuth DH (ed). Convenient and Rapid Analysis of GCMS, Hamburg, Germany
Macchioni, F., Cioni, P.L., Flamini, G., Morelli, I., Maccioni, S., Ansaldi, M. (2003). Chemical composition of essential oils from needles, branches and cones of Pinus pinea, P. halepensis, P. pinaster and P. nigra from central ltaly. Flavour and Fragrance Journal, 18,139-143.
McLafferty, F.W, Stauffer, D.B. (1989).The Wiley/NBS Registry of Mass Spectral Data, J Wiley and Sons: New York.
Nasri, N., Fady, B., Triki S. (2007). Quantification of Sterols and Aliphatic Alcohols in Mediterranean Stone Pine (Pinus pinea L.) Populations. Journal of Agriculture and Food Chemistry, 55, 2251-2255.
Nasri, N., Khaldi, A.,Fady, B., Triki S. (2005). Fatty acids from seeds of Pinus pinea L.: Composition and population profiling. Phytochemistry 66, 1729–1735.
Nasri, N., Tlili, N., Ammar, K.M., Khaldi, A.,Fady, B., Triki S. (2009). High tocopherol and triacylglycerol contents in Pinus pinea L. seeds. International Journal of Food Sciences and Nutrition. 60 (S1),161-169.
Nergiz, C., Dönmez, I. (2004). Chemical composition and nutritive value of Pinus pinea L. seeds. Food Chemistry. 86, 365–368.
Süntar, I., Tümen, I., Üstün, O., Keleş, H., Küpeli, A. E. (2012). Appraisal on the wound healing and anti-inflammatory activities of the essential oils obtained from the cones and needles of Pinus species by in vivo and in vitro experimental models. Journal of Ethnopharmacology, 139(2), 533-54
Üstün, O., Şenol, F.S, Kürkçüoğlu, M, Orhan, I.E, Kartal, M, Baser, K.H.C. (2012). Investigation on chemical composition, anticholinesterase and antioxidant activities of extracts and essential oils of Turkish Pinus species and pycnogenol. Industrial Crops and Products, 38, 115-23.
Cite
Bibtex | @ { nveo211768, journal = {Natural Volatiles \& Essential Oils}, issn = {}, eissn = {2148-9637}, address = {Badebio Biotechnology Ltd.}, year = {2015}, volume = {2}, pages = {39 – 44}, doi = {}, title = {Characterization and Antimicrobial Evaluation of the Essential Oil of Pinus pinea L. from Turkey}, key = {cite}, author = {Demirci, Fatih and Bayramiç, Pınar and Göger, Gamze and Demirci, Betül and Başer, Kemal} } |
APA | Demirci, F , Bayramiç, P , Göger, G , Demirci, B , Başer, K . (2015). Characterization and Antimicrobial Evaluation of the Essential Oil of Pinus pinea L. from Turkey. Natural Volatiles & Essential Oils, 2 (2), 39-44. Retrieved from http://dergipark.gov.tr/nveo/issue/19771/211768 |
MLA | Demirci, F , Bayramiç, P , Göger, G , Demirci, B , Başer, K . “Characterization and Antimicrobial Evaluation of the Essential Oil of Pinus pinea L. from Turkey”. Natural Volatiles & Essential Oils 2 (2015): 39-44 <http://dergipark.gov.tr/nveo/issue/19771/211768> |
Chicago | Demirci, F , Bayramiç, P , Göger, G , Demirci, B , Başer, K . “Characterization and Antimicrobial Evaluation of the Essential Oil of Pinus pinea L. from Turkey”. Natural Volatiles & Essential Oils 2 (2015): 39-44 |
RIS | TY – JOUR T1 – Characterization and Antimicrobial Evaluation of the Essential Oil of Pinus pinea L. from Turkey AU – Fatih Demirci , Pınar Bayramiç , Gamze Göger , Betül Demirci , Kemal Başer Y1 – 2015 PY – 2015 N1 – DO – T2 – Natural Volatiles & Essential Oils JF – Journal JO – JOR SP – 39 EP – 44 VL – 2 IS – 2 SN – -2148-9637 M3 – UR – Y2 – 2019 ER – |
EndNote | %0 Natural Volatiles & Essential Oils Characterization and Antimicrobial Evaluation of the Essential Oil of Pinus pinea L. from Turkey %A Fatih Demirci , Pınar Bayramiç , Gamze Göger , Betül Demirci , Kemal Başer %T Characterization and Antimicrobial Evaluation of the Essential Oil of Pinus pinea L. from Turkey %D 2015 %J Natural Volatiles & Essential Oils %P -2148-9637 %V 2 %N 2 %R %U |
ISNAD | Demirci, Fatih , Bayramiç, Pınar , Göger, Gamze , Demirci, Betül , Başer, Kemal . “Characterization and Antimicrobial Evaluation of the Essential Oil of Pinus pinea L. from Turkey”. Natural Volatiles & Essential Oils 2 / 2 (July 2015): 39-44. |
FULL TEXT: NVEO 2015, Volume 2, Issue 2, Pages 39-44