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2015 VOLUME 2, ISSUE 2, PAGES 45 – 51
Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles¥

Gökalp İşcan1, 2*, Betül Demirci1, Fatih Demirci1, 3 and K. Hüsnü Can Başer4

1Department of Pharmacognosy, Anadolu University, Faculty of Pharmacy, 26470, Eskişehir, Turkey.
2Yunus Emre Vocational School, Anadolu University, Eskişehir, Turkey.
3Faculty of Health Sciences, Anadolu University, Eskişehir, Turkey.
4Department of Botany and Microbiology, King Saud University, College of Science, Riyadh, Saudi Arabia.

*Corresponding author. Email: giscan@anadolu.edu.tr

FULL TEXT: NVEO 2015, Volume 2, Issue 2, Pages 45-51

Abstract

Geotrichum fragrans (syn. Saprochaete suaveolens) can produce fruity aromas (pine-apple like) such as esters and alcohols, when cultivated in glucose containing liquid media. Consumers’ preferences for the use of natural products especially in foods and cosmetics have motivated the production of aroma chemicals by biotechnological means. In the present study, the volatile compounds of the G. fragrans during the 9 days cultivation in liquid media were accumulated by Headspace- Solid Phase Microextraction (HS-SPME) technique, and, analysed by gas chromatography/flame ionisation detector (GC/FID) and GC coupled to mass spectrometry (GC/MS), simultaneously. The main volatiles of the G. fragrans culture were determined as ethyl tiglate (8.5-74.6%), ethyl isovalerate (36.1-41.6%) and methyl isovalerate (0.1-10.9%) during the time course. Daily variation trends of the aroma volatiles were determined, where ethyl acetate was postulated as an intermediate metabolite in this bioconversion pathway.

Keywords

Geotrichum fragrans, bioconversion, HS-SPME, GC-FID, GC/MS, aroma volatiles

References

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Joulain, D., & König, W. A. (1998). The Atlas of spectra data of sesquiterpene hydrocarbons. Hamburg, E.B.- Verlag,

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Mdaini, M., Gargouri, M., Hammami, M., Monser, L., & Hamdi, M. (2006). Production of Natural Fruity Aroma by Geotrichum candidum. Applied Biochemistry and Biotechnology, 128, 227-235.

Neto, R. S., Pastore, G. M. & Macedo, G.A. (2006). Biocatalysis and Biotransformation Producing γ- Decalactone. Food Science c, 69(9), 677-680.

Pinotti, T., Carvalho, P. M. B., Garcia, K. M. G., Silva, T. R., Hagler, A. N., Leite, & S. G. F. (2006). Media components and amino acid supplements influencing the production of fruity aroma by Geotrichum candidum. Brazilian Journal of Microbiology, 37, 494-498.

Cite
Bibtex @ { nveo211769, journal = {Natural Volatiles \& Essential Oils}, issn = {}, eissn = {2148-9637}, address = {Badebio Biotechnology Ltd.}, year = {2015}, volume = {2}, pages = {45 – 51}, doi = {}, title = {Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles}, key = {cite}, author = {İşcan, Gökalp and Demirci, Betül and Demirci, Fatih and Başer, Kemal} }
APA İşcan, G , Demirci, B , Demirci, F , Başer, K . (2015). Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles. Natural Volatiles & Essential Oils, 2 (2), 45-51. Retrieved from http://dergipark.gov.tr/nveo/issue/19771/211769
MLA İşcan, G , Demirci, B , Demirci, F , Başer, K . “Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles”. Natural Volatiles & Essential Oils 2 (2015): 45-51 <http://dergipark.gov.tr/nveo/issue/19771/211769>
Chicago İşcan, G , Demirci, B , Demirci, F , Başer, K . “Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles”. Natural Volatiles & Essential Oils 2 (2015): 45-51
RIS TY – JOUR T1 – Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles AU – Gökalp İşcan , Betül Demirci , Fatih Demirci , Kemal Başer Y1 – 2015 PY – 2015 N1 – DO – T2 – Natural Volatiles & Essential Oils JF – Journal JO – JOR SP – 45 EP – 51 VL – 2 IS – 2 SN – -2148-9637 M3 – UR – Y2 – 2019 ER –
EndNote %0 Natural Volatiles & Essential Oils Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles %A Gökalp İşcan , Betül Demirci , Fatih Demirci , Kemal Başer %T Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles %D 2015 %J Natural Volatiles & Essential Oils %P -2148-9637 %V 2 %N 2 %R %U
ISNAD İşcan, Gökalp , Demirci, Betül , Demirci, Fatih , Başer, Kemal . “Headspace Solid Phase Microextraction (HS-SPME) and Analysis of Geotrichum fragrans Volatiles”. Natural Volatiles & Essential Oils 2 / 2 (July 2015): 45-51.

FULL TEXT: NVEO 2015, Volume 2, Issue 2, Pages 45-51

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