Hanane Lougraimzi1*, Soufiane M’sou2, Abdelaaziz Bouaichi3, Imad Kotba3 and Achbani El Hassan4
1Laboratory of Nutrition, Health and Environment, Faculty of Sciences, Ibn Tofail University, Kenitra, MOROCCO
2Laboratory of Ecology and Environment and Applied Organic Chemistry Laboratory Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, MOROCCO
3Laboratory of Botany, Biotechnology and Plant Protection, Faculty of Sciences, Ibn Tofail University, Kenitra, MOROCCO
4Laboratory of Phytobacteriology and Biocontrol, National Institute of Agronomic Research, Meknes, MOROCCO
*Corresponding author. Email: hanane.lougraimzi@uit.ac.ma
FULL TEXT: NVEO 2020, Volume 7, Issue 2, Pages 35-43
Abstract
In the present study, in vitro antioxidant and antimicrobial activities of the essential oil of Pulicaria incisa (Lam.) DC., harvested from Oulad M’barek (Beni Mellal-Khenifra-Morocco) were evaluated. The essential oil obtained by hydrodistillation from the dried aerial parts was analysed by GC-MS. The major components were characterized as β-gurjunene (13.1%), β-bourbonene (11.5%), camphor (8.8%), γ-muurolene (7.9%), 1,8-cineole (eucalyptol) (6.1%), respectively. The P. incisa essential oil showed in the DPPH assay a value of IC50 105.99 ± 4.25 μg/mL and in the FRAP assay a value of EC50 87.21 ± 3.1 μg/mL, respectively. The antimicrobial activity of the oil was evaluated against five microorganisms using the in vitro paper disk diffusion for the determination of MIC and MBC values. The results obtained revealed different levels of sensitivity of the bacterial strains tested. The Allorhizobium vitis (S4) and Agrobacterium tumefaciens (C58) strains were the most sensitive to the investigated oil, with inhibition diameters >20 mm and a MIC and MBC of 0.25 (v/v) and 0.5 (v/v), respectively. Our results suggest that the essential oil of P. incisa contains bioactive compounds with antioxidant and antimicrobial properties supporting its traditional therapeutic use. This essential oil merits further study as a potential product for the management of phytopathogenic bacteria.
Keywords
Pulicaria incisa (Lam.) DC., Antioxidant activity, Antibacterial, Essential oil, Sensitivity.
References
Abd El-Gleel, W. and Hassanien, M. (2012). Antioxidant properties and lipid profile of Diplotaxis harra, Pulicaria incisa and Avicennia marina. Acta alimentaria, 41(2), 143-151.
Al-Hajj, N. Q. M. Wang, H. Gasmalla, M. A. Ma, C. Thabit, R. Rahman, M. R. T. and Tang, Y. (2014). Chemical composition and antioxidant activity of the essential oil of Pulicaria inuloides. Journal of Food and Nutrition Research, 2(5), 221-227.
Amer, M.M.A., Ramadan, M.F. and Abd El-Gleel, W. (2007): Impact of Pulicaria incisa, Diplotaxis harra and Avicennia marina as hypocholesterolemic agent. Dt. Lebensmittel Rdsch, 103, 320–327.
Asghari, G. Zahabi, F. Eskandarian, A. Yousefi, H. and Asghari, M. (2014). Chemical composition and leishmanicidal activity of Pulicaria gnaphalodes essential oil. Research Journal of Pharmacognosy, 1(4), 27-33.
Bouaichi, A. Benkirane, R. Habbadi, K., Benbouazza, A. and Achbani, E. H. (2015). Antibacterial activities of the essential oils from medicinal plants against the growth of Pseudomonas savastanoi pv. savastanoi causal agent of olive knot. The Journal of Agricultural Science, 8(12), 415.
Burits, M. Bucar, F. (2000). Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14, 323–328.
Chaib, F. Allali, H. Bennaceur, M. and Flamini, G. (2017). Chemical composition and antimicrobial activity of essential oils from the aerial parts of Asteriscus graveolens (FORSSK.) LESS. and Pulicaria incisa (LAM.) DC. Two Asteraceae herbs growing wild in the hoggar. Chemistry and Biodiversity, 14(8), e1700092.
Cuendet, M., Hostettmann, K., Potterat, O., and Dyatmiko, W. (1997). Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta, 80(4), 1144-1152.
Ghazanfar SA. Handbook of Arabian Medicinal Plants; CRC Press: Boca Raton, FL, USA, 1994.
Harkat-Madouri, L., Asma, B., Madani, K., Said, Z. B. O. S., Rigou, P., Grenier, D., … and Boulekbache-Makhlouf, L. (2015). Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Industrial Crops and Products, 78, 148-153.
Harififar, F. Moshafi. M.H. Mansouri. S.H. Khodashenas, M. Khoshnoodi, M. (2007). In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control, 18, 800–805.
Ibn Sina. EL-Kanoun fil-Tib. Halaby Co., Cairo, Egypte. Vol. 3, 1971.
Ismaili, H. L. Milella, S. Fkih-Tetouani, A. Ilidrissi, A. Camporese, S. Sosa, G. Altinier, R. Della Loggia and R. Aquino. (2004). In vivo topical anti-inflammatory and in vitro antioxidant activities of two extracts of Thymus satureioides leaves. Journal of Ethnopharmacology, 91: 31-36.
Elegami, A.A.B., Ishag, K.E., Mahmoud, E.N., Alfutuh, I.M.A. and Karim, E.I.A. (1994): Insecticidal activity of Pulicaria undulata oil. Fitoterapia, 65, 82–83.
El-Kamali, H.H. Ahmed, A.H. Mohammed, A.S., Yahia, A.A.M. El-Tayeb, I. and Ali, A.A. (1998): Antibacterial properties of essential oils from Nigella sativa seeds, Cymbopogon citratus leaves and Pulicaria undulata aerial parts. Fitoterapia, 69, 77–78.
Elmann, A. Telerman, A. Mordechay, S. Erlank, H. and Ofir, R. (2012). Antioxidant and astroprotective effects of a Pulicaria incisa infusion. Hindawi Publishing Corporation, Oxidative medicine and cellular longevity. Volume 2012, 10.
Ezoubeiri, A. Gadhi, C. A. Fdil, N. Benharref, A. Jana, M. and Vanhaelen, M. (2005). Isolation and antimicrobial activity of two phenolic compounds from Pulicaria odora L. Journal of Ethnopharmacology, 99(2), 287-292.
Kersters, K. De Ley, J. Sneath, P. H. A. and Sackin, M. (1973). Numerical taxonomic analysis of Agrobacterium. Microbiology, 78(2), 227-239.
Kirby, A. J. and Schmidt, R. J. (1997). The antioxidant activity of Chinese herbs for eczema and of placebo herbs. Journal of Ethnopharmacology, 56(2), 103-108.
Mansour, R.M.A, Ahmed, A.A. Melek, F.R. Saleh, N. A. M. (1990). The flavonoids of Pulicaria incisa. Fitoterapia, 61, 186–187.
Mossa, J. S., Hifnawy, M. S., Al-Yahya, M. A., Al-Meshal, I. A., and Mekkawi, A. G. (1987). Aromatic Plants of Saudi Arabia-Part 8-* GC/MS Analysis of Essential Oils of Pulicaria arabica and P. undulata. International Journal of Crude Drug Research, 25(2), 113-119.
Mustafa, A. M. Eldahmy, S. I. Caprioli, G. Bramucci, M. Quassinti, L. Lupidi, G. and Maggi, F. (2018). Chemical composition and biological activities of the essential oil from Pulicaria undulata (L.) CA Mey. Growing wild in Egypt. Natural Product Research, 1-5.
Nabiel, A.M.S. (2003): Global phytochemistry: the Egyptian experience. Phytochemistry, 63, 239–241.
Oyaizu, M. (1986). Studies on products of browning reaction. The Japanese Journal of Nutrition and Dietetics, 44(6), 307-315.
Popoff, M. Y. K. Kersters, M. Kiredjian, I. Miras and C. Coynault. (1984). Taxonomic position of Agrobacterium strains of hospital origin. Annals of the Pasteur Institute / Microbiology, 135, 427-442.
Ramadan, M. F. Amer, M. M. A. Mansour, H. T. Wahdan, K. M. El-Sayed, R. M. El-Sanhoty, S. and El-Gleel, W. A. (2009). Bioactive lipids and antioxidant properties of wild Egyptian Pulicaria incisa, Diplotaxis harra, and Avicennia marina. Journal für Verbraucherschutz und Lebensmittelsicherheit, 4(3-4), 239.
Remmal, A. T. Bouchikhi, K. Rhayour, M. Ettayebi and A. Tantaoui-Elaraki. (1993). Improved method for the determination of antimicrobial activity of essential oils in agar medium. Journal of Essential Oil Research, 5, 179-184.
Rustaiyan, A. Habibi, Z. Saberi, M. and Jakupovic, J. (1991): A nor-guaianolide and a glaucolide-like eudesmanolide from Pulicaria undulata. Phytochemistry, 30(7), 2405–2406.
Ravandeh, M. Valizadeh, J. Noroozifar, M. and Khorasani-Motlagh, M. (2011). Screening of chemical composition of essential oil, mineral elements and antioxidant activity in Pulicaria undulata (L.) CA Mey from Iran. Journal of Medicinal Plants Research, 5(10), 2035-2040.
Sahin, F. Güllüce, M. Daferera, D. Sökmen, A. Sökmen, M. Polissiou, M. (2004). Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of Turkey. Food Control 15, 549–557.
Shabana, M. M. Mirhom, Y. W. Genenah, A. A. Aboutabl, E. A. and Amer, H. A. (1990): Study into wild Egyptian plants of potential medicinal activity. Ninth communication: hypoglycaemic activity of some selected plants in normal fasting and alloxanised rats. Archiv fur Experimentelle Veterinarmedizin, 44(3), 389-394.
Shahat, E. A. Bakr, R. O. Eldahshan, O. A. and Ayoub, N. A. (2017). Chemical Composition and Biological Activities of the Essential Oil from Leaves and Flowers of Pulicaria incisa subsp. candolleana (Family Asteraceae). Chemistry and Biodiversity, 14(4), e1600156.
Saleh, N. A. M. (2003). “Global phytochemistry: the Egyptian experience. Phytochemistry, 63(3), 239–241.
Williams, C.A, Harborne, J.B. Greenham, J. R. Grayer, R.J. Kite, G.G. Eagles J. (2003): Variation in lipophilic and vacuolar flavonoids among European Pulicaria species. Phytochemistry, 64, 275-283.
Cite
| Bibtex | @araştırma makalesi { nveo746549, journal = {Natural Volatiles and Essential Oils}, issn = {}, eissn = {2148-9637}, address = {}, publisher = {Badebio Biotechnology Ltd.}, year = {2020}, volume = {7}, pages = {35 – 43}, doi = {10.37929/nveo.746549}, title = {Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil}, key = {cite}, author = {Lougraımzı, Hanane and M’sou, Soufiane and Bouai̇chi̇, Abdelaaziz and Qotba, Imad and Achbani̇, El Hassan} } |
| APA | Lougraımzı, H , M’sou, S , Bouai̇chi̇, A , Qotba, I , Achbani̇, E . (2020). Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil . Natural Volatiles and Essential Oils , 7 (2) , 35-43 . DOI: 10.37929/nveo.746549 |
| MLA | Lougraımzı, H , M’sou, S , Bouai̇chi̇, A , Qotba, I , Achbani̇, E . “Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil” . Natural Volatiles and Essential Oils 7 (2020 ): 35-43 <https://dergipark.org.tr/tr/pub/nveo/issue/56007/746549> |
| Chicago | Lougraımzı, H , M’sou, S , Bouai̇chi̇, A , Qotba, I , Achbani̇, E . “Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil”. Natural Volatiles and Essential Oils 7 (2020 ): 35-43 |
| RIS | TY – JOUR T1 – Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil AU – Hanane Lougraımzı , Soufiane M’sou , Abdelaaziz Bouai̇chi̇ , Imad Qotba , El Hassan Achbani̇ Y1 – 2020 PY – 2020 N1 – doi: 10.37929/nveo.746549 DO – 10.37929/nveo.746549 T2 – Natural Volatiles and Essential Oils JF – Journal JO – JOR SP – 35 EP – 43 VL – 7 IS – 2 SN – -2148-9637 M3 – doi: 10.37929/nveo.746549 UR – https://doi.org/10.37929/nveo.746549 Y2 – 2020 ER – |
| EndNote | %0 Natural Volatiles and Essential Oils Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil %A Hanane Lougraımzı , Soufiane M’sou , Abdelaaziz Bouai̇chi̇ , Imad Qotba , El Hassan Achbani̇ %T Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil %D 2020 %J Natural Volatiles and Essential Oils %P -2148-9637 %V 7 %N 2 %R doi: 10.37929/nveo.746549 %U 10.37929/nveo.746549 |
| ISNAD | Lougraımzı, Hanane , M’sou, Soufiane , Bouai̇chi̇, Abdelaaziz , Qotba, Imad , Achbani̇, El Hassan . “Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil”. Natural Volatiles and Essential Oils 7 / 2 (Haziran 2020): 35-43 . https://doi.org/10.37929/nveo.746549 |
| AMA | Lougraımzı H , M’sou S , Bouai̇chi̇ A , Qotba I , Achbani̇ E . Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil. Nat. Volatiles & Essent. Oils. 2020; 7(2): 35-43. |
| Vancouver | Lougraımzı H , M’sou S , Bouai̇chi̇ A , Qotba I , Achbani̇ E . Evaluation of the antioxidant and antibacterial activity of Pulicaria incisa (Lam.) essential oil. Natural Volatiles and Essential Oils. 2020; 7(2): 35-43. |
FULL TEXT: NVEO 2020, Volume 7, Issue 2, Pages 35-43
